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KMID : 0624620080410020158
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BMB Reports
2008 Volume.41 No. 2 p.158 ~ p.163
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Hepatitis B virus X protein enhances NFκB activity through cooperating with VBP1
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Kim Sang-Yong
Kim Jin-Chul
Kim Jeong-Ki
Kim Hye-Jin
Lee Hee-Min
Choi Mi-Sun
Maeng Pil-Jae
Ahn Jeong-Keun
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Abstract
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Hepatitis B virus X protein (HBx) is essential for hepatitis B virus infection and exerts a pleiotropic effect on various cellular machineries. HBx has been also demonstrated as an indirect transcriptional transactivator of various different viral and cellular promoters. In addition, HBx is involved in the development of various liver diseases including hepatocellular carcinoma. However the mechanism of HBx in hepatocellular carcinogenesis remains largely unknown. In this study, to identify possible new cellular proteins interacting with HBx, we carried out yeast two-hybrid assay. We obtained several possible cellular partners including VBP1, a binding factor for VHL tumor suppressor protein. The direct physical interaction between HBx and VBP1 in vitro and in vivo was confirmed by immunoprecipitation assay. In addition, we found that VBP1 facilitates HBx-induced NF&kgr;B activation and cell proliferation. These results implicate the important role of HBx in the development of hepatocellular carcinoma through its interaction with VBP1. [BMB reports 2008; 41(2): 158-163]
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KEYWORD
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Cell proliferation, HBV, HBx, NF&kgr, B, VBP1, VHL
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